منابع مشابه
DNA primase activity associated with DNA polymerase alpha from Xenopus laevis ovaries.
One of the two forms of DNA polymerase alpha from ovaries of the frog Xenopus laevis catalyzed ribonucleoside triphosphate-dependent DNA synthesis on single-stranded circular fd phage DNA templates. DNA synthesis was dependent on ATP and added template. CTP, GTP, and UTP stimulated DNA synthesis but were not required and could not substitute for ATP. DNA synthesis was not inhibited by alpha-ama...
متن کاملA low molecular weight DNA polymerase from ovaries of the frog Xenopus laevis. DNA polymerase-beta (ovarian).
A low molecular weight DNA polymerase (DNA nucleotidyltransferase; EC 2.7.7.7) has been purified 265,000-fold from ovaries of the frogXertopus Zuevis. On polyacrylamide gels run under denaturing conditions the purified preparation exhibited one major band of approximately 45,000 molecular weight. The most purified fraction incorporated 380 pmol of dTMP/h/mg of protein at 26”. No endodeoxyribonu...
متن کاملA Low Molecular Weight DNA Polymerase from Ovaries of the Frog Xenopus laevis
A low molecular weight DNA polymerase (DNA nucleotidyltransferase; EC 2.7.7.7) has been purified 265,000-fold from ovaries of the frogXertopus Zuevis. On polyacrylamide gels run under denaturing conditions the purified preparation exhibited one major band of approximately 45,000 molecular weight. The most purified fraction incorporated 380 pmol of dTMP/h/mg of protein at 26”. No endodeoxyribonu...
متن کاملFormation of extrachromosomal circles from telomeric DNA in Xenopus laevis.
Instability and plasticity of telomeric DNA, which includes extrachromosomal DNA, are usually correlated with the absence of telomerase and with abnormal growth of mammalian cells. Here, we show the formation of extrachromosomal circular DNA of telomeric repeats (tel-eccDNA) during the development of Xenopus laevis. Tel-eccDNA is double-stranded relaxed circles composed of the vertebrate consen...
متن کاملNucleolar Dna in Oocytes of Xenopus Laevis
The ovaries of newly metamorphosed Xenopus females contain oocytes in all stages of early meiotic prophase. In pachytene nuclei extrachromosomal nucleolar DNA appears in the form of a thin cap covering one side of the nucleus. During pachytene this cap of DNA enlarges to occupy half the nucleus. After pachytene the nucleus grows rapidly and the cap of DNA disperses into numerous tiny granules w...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1989
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(20)88212-x